The regulation of host defences to infection by the microbiota

The skin and mucosal epithelia of humans and other mammals are permanently colonised by large microbial communities (the microbiota). Due to this life-long association with the microbiota, these microbes have an extensive influence over the physiology of their host organism. It is now becoming apparent that nearly all tissues and organ systems, whether in direct contact with the microbiota, or in deeper host sites, are under microbial influence. The immune system is perhaps the most profoundly affected, with the microbiota programming both its innate and adaptive arms. The regulation of immunity by the microbiota helps protect the host against intestinal and extra-intestinal infection by many classes of pathogen. In this review, we will discuss the experimental evidence supporting a role for the microbiota in regulating host defences to extra-intestinal infection, draw together common mechanistic themes, including the central role of pattern recognition receptors, and outline outstanding questions which need to be answered. This article is protected by copyright. All rights reserved

Overcoming the barriers to walking for children

This paper is an output from the project CAPABLE (Children?s Activities,Perceptions and Behaviour in the Local Environment) being carried out at UCL,jointly between the Centre for Transport Studies, the Department of Psychology, theBartlett School of Planning and the Centre for Advanced Spatial Analysis. The overallaim of the project, which runs from 1 August 2004 to 31 July 2006, is to examine theinteraction between children and the local environment, including identifying howchildren use open space and streets, and why they go to some places but not others.This paper draws on results from questionnaires completed by children about theextent to which they are allowed out unaccompanied by an adult. The surveys werecarried out in four schools, two in Hertfordshire, the area immediately north ofLondon, and two in the London Borough of Lewisham. The purpose is to establish theextent to which the children are allowed by their parents to go out unaccompanied byadults. The issues covered include whether the children go out walking or cyclingwithout an adult, whether they are allowed out alone to visit friends houses, go outafter dark or to cross main roads. The results are considered in terms of the children?sage and gender, and in terms of the households? car ownership level and the strengthof its local social networks. It is found that more of the children in Hertfordshire areallowed out alone, despite the fact that the factors that seem to correlate with beingallowed out unaccompanied are stronger in Lewisham. It is concluded that this maywell be due to environmental factors, real and perceived

Setting Children Free: Children’s Independent Movement in the Local Environment

Parental concerns about children’s safety and security are restricting children’s independent exploration of the local environment. Children are being denied important opportunities to exercise, to acquire decision-making skills, such as crossing the road safely, and to develop social skills through interaction with their peers. This paper presents findings from the project CAPABLE (Children’s Activities, Perceptions And Behaviour in the Local Environment) being carried out at University College London. Based on findings from fieldwork carried out with children aged 8-11 in Cheshunt, Hertfordshire, the paper shows the effect of factors such as the number of adults at home, having an older sibling, having a car or garden at home and living near to a park on the propensity to be allowed out alone. Then it considers how being allowed out alone affects the amount of time children spend outdoors, playing with friends and watching television. The paper then uses data from children who have been fitted with physical activity monitors and GPS (Global Positioning Satellite) monitors and asked to keep diaries, to show how children’s travel behaviour differs when they are with adults from when they are not

Calculating the random guess scores of multiple-response and matching test items

For achievement tests, the guess score is often used as a baseline for the lowest possible grade for score to grade transformations and setting the cut scores. For test item types such as multiple-response, matching and drag-and-drop, determin-ing the guess score requires more elaborate calculations than the more straight-forward calculation of the guess score for True-False and multiple-choice test item formats. For various variants of multiple-response and matching types with respect to dichotomous and polytomous scoring, methods for determining the guess score are presented and illustrated with practical applications. The implica-tions for theory and practice are discussed

Correlates of genetic monogamy in socially monogamous mammals: insights from Azara's owl monkeys

Understanding the evolution of mating systems, a central topic in evolutionary biology for more than 50 years, requires examining the genetic consequences of mating and the relationships between social systems and mating systems. Among pair-living mammals, where genetic monogamy is extremely rare, the extent of extra-group paternity rates has been associated withmale participation in infant care, strength of the pair bond and length of the breeding season. This study evaluated the relationship between two of those factors and the genetic mating system of socially monogamous mammals, testing predictions that male care and strength of pair bond would be negatively correlated with rates of extra-pair paternity (EPP). Autosomal microsatellite analyses provide evidence for genetic monogamy in a pair-living primate with bi-parental care, the Azara’s owl monkey (Aotus azarae). A phylogenetically corrected generalized least square analysis was used to relate male care and strength of the pair bond to their genetic mating system (i.e. proportions of EPP) in 15 socially monogamous mammalian species. The intensity of male care was correlated with EPP rates in mammals, while strength of pair bond failed to reach statistical significance. Our analyses showthat, once social monogamy has evolved, paternal care, and potentially also close bonds, may facilitate the evolution of genetic monogamy.German Science Foundation (HU 1746/2-1); Wenner-Gren Foundation; L.S.B. Leakey Foundation;National Geographic Society; National Science Foundation (BCS-0621020, 1219368, and 1232349); the University of Pennsylvania Research Foundation; the Zoological Society of San Dieg

Transport, Physical Activity and Health: Present knowledge and the way ahead

The objective of this report is to consider the available evidence on the role of transport in increasing levels of physical activity, and so contribute to addressing the nation’s health problems caused by low levels of physical activity. Evidence on the links between transport and physical activity is examined, followed by examination of the effects of individual behaviour on physical activity. Whilst research on this topic has had some impact, there are a number on concerns about the approach, in particular the lack of evidence on long-term effects. Modifications to the built environment to increase physical activity are discussed: these need to be supported by other measures to be fully effective. The key relationship is between car use and physical activity. In order to increase levels of physical activity, it is necessary to reduce use of the car. Because so many households have adopted lifestyles that revolve around the use of the car, it is important to recognise that any policies to reduce car use must provide as much, or close to as much, accessibility as the car does. One way to do this is to shift the method of accessing cars from individual household ownership to a more flexible system of hiring or sharing cars. There would need to be a variety of supporting policy actions affecting transport and land use. It would be very useful to have a wide debate about transport modelling to ensure that the models represent travel and locational behaviour effectively, including factors that represent physical activity; the discussion should include the cost effectiveness of making changes to the existing modelling system. The implementation of the approach presented in the report could lead to significant improvements in the health of the nation and cost savings to the National Health Service

Negative impact of the first COVID-19 lockdown upon health-related behaviours and psychological wellbeing in people living with severe and complex obesity in the UK

BACKGROUND: Coronavirus disease 2019 (COVID-19) has led to unprecedented changes in the way we live, particularly for people at higher risk of severe illness from COVID-19. People with pre-existing health conditions have been markedly impacted and, in some instances, left unsupported due to reduced provision of routine healthcare services. People living with obesity (PLWO) are identified as at higher risk of severe illness from COVID-19 infection. Currently, there is a paucity of evidence about the impact of the first COVID-19 lockdown on PLWO, including those accessing weight management and bariatric surgery services (WMS). METHODS: 543 adults (16–80 years) with obesity (BMI ≥ 30 kg/m2) were recruited between 14th May and 9th July 2020 through social media advertisements, professional and patient obesity organisations and WMS. Participants completed an online survey regarding the impact of the first COVID-19 lockdown upon, mental health, well-being, health-related behaviours, risk mitigating behaviours, access to WMS and weight stigma. FINDINGS: During the first COVID-19 lockdown, the majority of PLWO reported deterioration of their mental health and health-related behaviours such as diet, physical activity (PA) and sleep. With 55% reporting an unhealthier diet, 61% reduced PA and 80% worsening of their sleep. Higher depression and lower wellbeing scores were found to associate with the greatest adverse impact upon health-related behaviours. PLWO who were attending WMS prior to the first lockdown reported a greater deterioration of their diet, with nearly 50% reporting worsening of their diet and PA worsening compared to PLWO who were not attending WMS. Most participants took two or more risk mitigating actions (73%). PLWO attending WMS reported reduced access (44%) with insufficient information (49%) from their clinical service providers. The majority of participants reported no change in perceived weight stigma. INTERPRETATION: This study shows the detrimental impact of the first COVID-19 lockdown on PLWO in relation to health-related behaviours, mental health and access to WMS. Our findings show that PLWO with poor mental health and those attending WMS were most adversely impacted and highlights the need for greater mental health support and continued provision of support from WMS for PLWO during future lockdowns. FUNDING: This research was funded through National Institute for Health Research University College London Hospitals Biomedical Research Centre funding

Signs of deterioration in infants discharged home following congenital heart surgery in the first year of life: a qualitative study

AIMS: To describe the ways in which parents recognise and make decisions about their child's symptoms following discharge home after congenital heart interventions in the first year of life and their experiences of seeking help. METHODS: This was a qualitative study involving semistructured interviews with parents. Twenty-one parents were recruited to the study. Parents all had a child who had congenital heart surgery in their first year of life between September 2009 and October 2013 at one of three UK cardiac centres; the children had either died or were readmitted as an emergency following initial discharge. RESULTS: Some parents were unable to identify any early warning signs. Others described symptoms of deterioration including changes in feeding and appearance, respiratory distress and subtle behavioural changes that may not be routinely highlighted to parents at discharge. Several barriers to accessing prompt medical assistance were identified including parents feeling that their concerns were not taken seriously, long wait times and lack of protocols at A&E. CONCLUSIONS: Our study highlights behavioural symptoms as being a potentially underemphasised sign of deterioration and identifies a number of barriers to parents accessing support when they are concerned. It is important that parents are encouraged to seek advice at the earliest opportunity and that those health professionals at the front line have access to the information they need in order to respond in an appropriate and timely way. A role for home monitoring was also noted as potentially useful in identifying at risk children who appear clinically well

Molecular Gas in Quasar Hosts

The study of molecular gas in quasar host galaxies addresses a number of interesting questions pertaining to the hosts' ISM, to unified schemes relating quasars and IR galaxies, and to the processes fueling nuclear activity. In this contribution I review observations of molecular gas in quasar hosts from z=0.06 to z=4.7. The Cloverleaf quasar at z=2.5 is featured as a case where there are now enough detected transitions (four in CO, and one each in CI and HCN) to allow detailed modeling of physical conditions in the molecular ISM. We find that the CO-emitting gas is warmer, denser, and less optically thick than that found in typical Galactic molecular clouds. These differences are probably due to the presence of the luminous quasar in the nucleus of the Cloverleaf's host galaxy